Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mol Biol Cell
2000 Jan 01;111:201-15. doi: 10.1091/mbc.11.1.201.
Show Gene links
Show Anatomy links
The Rib43a protein is associated with forming the specialized protofilament ribbons of flagellar microtubules in Chlamydomonas.
Norrander JM
,
deCathelineau AM
,
Brown JA
,
Porter ME
,
Linck RW
.
???displayArticle.abstract???
Ciliary and flagellar microtubules contain a specialized set of three protofilaments, termed ribbons, that are composed of tubulin and several associated proteins. Previous studies of sea urchin sperm flagella identified three of the ribbon proteins as tektins, which form coiled-coil filaments in doublet microtubules and which are associated with basal bodies and centrioles. To study the function of tektins and other ribbon proteins in the assembly of flagella and basal bodies, we have begun an analysis of ribbons from the unicellular biflagellate, Chlamydomonas reinhardtii, and report here the molecular characterization of the ribbon protein rib43a. Using antibodies against rib43a to screen an expression library, we recovered a full-length cDNA clone that encodes a 42,657-Da polypeptide. On Northern blots, the rib43a cDNA hybridized to a 1. 7-kb transcript, which was up-regulated upon deflagellation, consistent with a role for rib43a in flagellar assembly. The cDNA was used to isolate RIB43a, an approximately 4.6-kb genomic clone containing the complete rib43a coding region, and restriction fragment length polymorphism analysis placed the RIB43a gene on linkage group III. Sequence analysis of the RIB43a gene indicates that the substantially coiled-coil rib43a protein shares a high degree of sequence identity with clones from Trypanosoma cruzi and Homo sapiens (genomic, normal fetal kidney, and endometrial and germ cell tumors) but little sequence similarity to other proteins including tektins. Affinity-purified antibodies against native and bacterially expressed rib43a stained both flagella and basal bodies by immunofluorescence microscopy and stained isolated flagellar ribbons by immuno-electron microscopy. The structure of rib43a and its association with the specialized protofilament ribbons and with basal bodies is relevant to the proposed role of ribbons in forming and stabilizing doublet and triplet microtubules and in organizing their three-dimensional structure.
Afzelius,
Situs inversus and ciliary abnormalities. What is the connection?
1996, Pubmed
Afzelius,
Situs inversus and ciliary abnormalities. What is the connection?
1996,
Pubmed
Allen,
Structural polarity and directional growth of microtubules of Chlamydomonas flagella.
1975,
Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Amos,
Studies of tektin filaments from flagellar microtubules by immunoelectron microscopy.
1987,
Pubmed
,
Echinobase
Amos,
Arrangement of subunits in flagellar microtubules.
1974,
Pubmed
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
Baccetti,
The biology of the sperm cell.
1976,
Pubmed
Cavalier-Smith,
Basal body and flagellar development during the vegetative cell cycle and the sexual cycle of Chlamydomonas reinhardii.
1975,
Pubmed
Chen,
Tektin B1 from ciliary microtubules: primary structure as deduced from the cDNA sequence and comparison with tektin A1.
1994,
Pubmed
,
Echinobase
Chen,
Mutation of the mouse hepatocyte nuclear factor/forkhead homologue 4 gene results in an absence of cilia and random left-right asymmetry.
1998,
Pubmed
Cole,
Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons.
1998,
Pubmed
Collet,
Analysis of osm-6, a gene that affects sensory cilium structure and sensory neuron function in Caenorhabditis elegans.
1998,
Pubmed
Davies,
Sequences controlling transcription of the Chlamydomonas reinhardtii beta 2-tubulin gene after deflagellation and during the cell cycle.
1994,
Pubmed
Dutcher,
Flagellar assembly in two hundred and fifty easy-to-follow steps.
1995,
Pubmed
Dutcher,
The UNI3 gene is required for assembly of basal bodies of Chlamydomonas and encodes delta-tubulin, a new member of the tubulin superfamily.
1998,
Pubmed
Ehler,
Loss of spatial control of the mitotic spindle apparatus in a Chlamydomonas reinhardtii mutant strain lacking basal bodies.
1996,
Pubmed
Fowkes,
The role of preassembled cytoplasmic complexes in assembly of flagellar dynein subunits.
1999,
Pubmed
Gardner,
Components of a "dynein regulatory complex" are located at the junction between the radial spokes and the dynein arms in Chlamydomonas flagella.
1994,
Pubmed
Gibbons,
Cilia and flagella of eukaryotes.
1982,
Pubmed
Gibbons,
Relationship between the latent adenosine triphosphatase state of dynein 1 and its ability to recombine functionally with KCl-extracted sea urchin sperm flagella.
1979,
Pubmed
,
Echinobase
Goodenough,
Substructure of inner dynein arms, radial spokes, and the central pair/projection complex of cilia and flagella.
1985,
Pubmed
,
Echinobase
Goodenough,
Outer and inner dynein arms of cilia and flagella.
1985,
Pubmed
,
Echinobase
Goodenough,
BALD-2: a mutation affecting the formation of doublet and triplet sets of microtubules in Chlamydomonas reinhardtii.
1975,
Pubmed
Haimo,
Dynein binds to and crossbridges cytoplasmic microtubules.
1980,
Pubmed
Hinchcliffe,
Two proteins isolated from sea urchin sperm flagella: structural components common to the stable microtubules of axonemes and centrioles.
1998,
Pubmed
,
Echinobase
Hoober,
The Chlamydomonas Sourcebook. A Comprehensive Guide to Biology and Laboratory Use. Elizabeth H. Harris. Academic Press, San Diego, CA, 1989. xiv, 780 pp., illus. $145.
2010,
Pubmed
Huang,
Paralyzed flagella mutants of Chlamydomonas reinhardtii. Defective for axonemal doublet microtubule arms.
1979,
Pubmed
Huang,
Radial spokes of Chlamydomonas flagella: genetic analysis of assembly and function.
1981,
Pubmed
James,
A mutation in the alpha 1-tubulin gene of Chlamydomonas reinhardtii confers resistance to anti-microtubule herbicides.
1994,
Pubmed
Keil,
Functional morphology of insect mechanoreceptors.
1998,
Pubmed
King,
Purification and characterization of Chlamydomonas flagellar dyneins.
1987,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lefebvre,
Flagellar amputation and regeneration in Chlamydomonas.
1995,
Pubmed
Linck,
Characterization of antibodies as probes for structural and biochemical studies of tektins from ciliary and flagellar microtubules.
1988,
Pubmed
,
Echinobase
Linck,
Localization of tektin filaments in microtubules of sea urchin sperm flagella by immunoelectron microscopy.
1985,
Pubmed
,
Echinobase
Lupas,
Prediction and analysis of coiled-coil structures.
1996,
Pubmed
Lupas,
Predicting coiled coils from protein sequences.
1991,
Pubmed
Mastronarde,
Arrangement of inner dynein arms in wild-type and mutant flagella of Chlamydomonas.
1992,
Pubmed
Meza,
Chemical heterogeneity of protofilaments forming the outer doublets from sea urchin flagella.
1973,
Pubmed
,
Echinobase
Mochizuki,
Cloning of inv, a gene that controls left/right asymmetry and kidney development.
1998,
Pubmed
Myster,
The Chlamydomonas Dhc1 gene encodes a dynein heavy chain subunit required for assembly of the I1 inner arm complex.
1997,
Pubmed
Nelson,
The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation.
1994,
Pubmed
Nojima,
At least one of the protofilaments in flagellar microtubules is not composed of tubulin.
1995,
Pubmed
,
Echinobase
Nonaka,
Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein.
1999,
Pubmed
Norrander,
Expression of ciliary tektins in brain and sensory development.
1998,
Pubmed
Norrander,
Structural comparison of tektins and evidence for their determination of complex spacings in flagellar microtubules.
1996,
Pubmed
,
Echinobase
Norrander,
Primary structure of tektin A1: comparison with intermediate-filament proteins and a model for its association with tubulin.
1992,
Pubmed
,
Echinobase
Norrander,
Transcriptional control of tektin A mRNA correlates with cilia development and length determination during sea urchin embryogenesis.
1995,
Pubmed
,
Echinobase
Norrander,
Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
1984,
Pubmed
Perkins,
Mutant sensory cilia in the nematode Caenorhabditis elegans.
1986,
Pubmed
Piperno,
Two-dimensional analysis of flagellar proteins from wild-type and paralyzed mutants of Chlamydomonas reinhardtii.
1977,
Pubmed
Piperno,
Three distinct inner dynein arms in Chlamydomonas flagella: molecular composition and location in the axoneme.
1990,
Pubmed
Pirner,
Tektins are heterodimeric polymers in flagellar microtubules with axial periodicities matching the tubulin lattice.
1995,
Pubmed
,
Echinobase
Pirner,
Methods for the isolation of tektins and Sarkosyl-insoluble protofilament ribbons.
1995,
Pubmed
,
Echinobase
Porter,
The dynein gene family in Chlamydomonas reinhardtii.
1997,
Pubmed
Porter,
Axonemal dyneins: assembly, organization, and regulation.
1996,
Pubmed
Ranum,
Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms.
1989,
Pubmed
Schnell,
Isolation of the Chlamydomonas regulatory gene NIT2 by transposon tagging.
1993,
Pubmed
Silflow,
The two alpha-tubulin genes of Chlamydomonas reinhardi code for slightly different proteins.
1986,
Pubmed
Silflow,
Molecular mapping of genes for flagellar proteins in Chlamydomonas.
1995,
Pubmed
Smith,
Structural and functional reconstitution of inner dynein arms in Chlamydomonas flagellar axonemes.
1992,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1976,
Pubmed
Srivastava,
Left, right ... which way to turn?
1997,
Pubmed
Steffen,
Evidence for tektins in centrioles and axonemal microtubules.
1988,
Pubmed
,
Echinobase
Steffen,
Relationship between tektins and intermediate filament proteins: an immunological study.
1990,
Pubmed
,
Echinobase
Steffen,
Centrosomal components immunologically related to tektins from ciliary and flagellar microtubules.
1995,
Pubmed
,
Echinobase
Stephens,
Retention of ciliary ninefold structure after removal of microtubules.
1990,
Pubmed
,
Echinobase
Stephens,
Tektins as structural determinants in basal bodies.
1998,
Pubmed
Takada,
Functional reconstitution of Chlamydomonas outer dynein arms from alpha-beta and gamma subunits: requirement of a third factor.
1994,
Pubmed
Tilney,
Microtubules: evidence for 13 protofilaments.
1975,
Pubmed
,
Echinobase
Warner,
The structural basis of ciliary bend formation. Radial spoke positional changes accompanying microtubule sliding.
1975,
Pubmed
Wilkerson,
Molecular analysis of the gamma heavy chain of Chlamydomonas flagellar outer-arm dynein.
1994,
Pubmed
Witman,
Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes.
1972,
Pubmed
Witman,
Chlamydomonas flagella. II. The distribution of tubulins 1 and 2 in the outer doublet microtubules.
1972,
Pubmed
Witman,
Isolation of Chlamydomonas flagella and flagellar axonemes.
1987,
Pubmed
Wright,
A nucleus-basal body connector in Chlamydomonas reinhardtii that may function in basal body localization or segregation.
1985,
Pubmed
Young,
Yeast RNA polymerase II genes: isolation with antibody probes.
1983,
Pubmed
