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Nucleic Acids Res
1979 Dec 11;77:1787-98. doi: 10.1093/nar/7.7.1787.
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Isolation of a genomal clone containing chicken histone genes.
Harvey RP
,
Wells JR
.
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We have used enriched chicken histone cDNA to select genomal clones from a chicken library. Because the cDNA probe also contained other sequences, a further screening of positive plagues with negative probes eliminated most non-histone gene clones. One ''positively-selected'' genomal clone, lambda CH-01, hybridised with cloned sea-urchin histone genes and also detected histone genes in EcoRI-digested genomal sea-urchin DNA. Limited DNA sequencing of HaeIII fragments identified two sequences within the coding region of chicken histone H2A. A third fragment predicted an amino acid sequence with strong homology to an H1 histone sequence.
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