ECB-ART-38541
J Cell Biol
1962 Dec 01;15:503-8. doi: 10.1083/jcb.15.3.503.
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The isolation of nuclei from eggs and embryos of the sea urchin.
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NUCLEI CAN BE ISOLATED FROM THE EGGS AND EMBRYOS OF THE SEA URCHIN BY A FOUR STEP METHOD: (1) washing with dextrose solution until the cells become weak, (2) cytolysis in 0.002 M Mg(++) solution, (3) homogenization of the cytolyzed cells by agitation, and (4) separation of the nuclei from the rest of the homogenate by centrifugation on a layered sucrose gradient. Contamination of the isolated nuclei is less than 1 per cent. The yield varies between 20 per cent and 80 per cent depending on the developmental stage used.
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Genes referenced: LOC100887844
References [+] :
AFZELIUS,
The ultrastructure of the nuclear membrane of the sea urchin oocyte as studied with the electron microscope.
2003, Pubmed,
Echinobase