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Chromosoma
1982 Jan 01;852:251-60. doi: 10.1007/bf00294969.
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Supranucleosomal organization of chromatin. Electron microscopic visualization of long polynucleosomal chains.
Azorín F
,
Pérez-Grau L
,
Subirana JA
.
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A systematic study of the effect of different ionic conditions on the morphology of the 25-30 nm chromatin fiber from chicken erythrocytes has revealed that, as the ionic strength is increased, knobby fibers with a clear superbead structure are formed in the presence of either Mg++ or Na+, or both. A further increase in ionic strength results in smooth chromatin fibers due to a tight packing of superbeads. Cross-linking such fibers with formaldehyde and reversal of the ionic conditions, demonstrates the superbead structures underlying the smooth fibers observed at high ionic concentrations. The average size of the superbeads is 34 nm along the length of the fibers, in agreement with the value found in embedded sea cucumber chromatin. A second class of superbeads has an average length of 25 nm and probably corresponds to partially disrupted structures.
Bustin,
Chromatin structure visualization by immunoelectron microscopy.
1976, Pubmed
Bustin,
Chromatin structure visualization by immunoelectron microscopy.
1976,
Pubmed
Finch,
Electron microscopy of defined lengths of chromatin.
1976,
Pubmed
Finch,
Solenoidal model for superstructure in chromatin.
1976,
Pubmed
Gall,
Chromosome fibers studied by a spreading technique.
1967,
Pubmed
Griffith,
Chromatin structure: deduced from a minichromosome.
2010,
Pubmed
Hozier,
The chromosome fiber: evidence for an ordered superstructure of nucleosomes.
1977,
Pubmed
Itkes,
Repeating oligonucleosomal units. A new element of chromatin structure.
1981,
Pubmed
Jorcano,
Aggregation of small oligonucleosomal chains into 300-A globular particles.
1981,
Pubmed
Loening,
The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
1967,
Pubmed
Noll,
Preparation of native chromatin and damage caused by shearing.
2010,
Pubmed
Olins,
Chromatin nu bodies: isolation, subfractionation and physical characterization.
1977,
Pubmed
Pruitt,
A repeating unit of higher order chromatin structure in chick red blood cell nuclei.
1980,
Pubmed
Renz,
Involvement of histone H1 in the organization of the chromosome fiber.
1977,
Pubmed
Renz,
Heterogeneity of the chromosome fiber.
1979,
Pubmed
Ris,
Chromosome structure.
1973,
Pubmed
Strätling,
The higher order repeat structure of chromatin is built up of globular particles containing eight nucleosomes.
1979,
Pubmed
Subirana,
The subunit structure of chromatin fibres.
1981,
Pubmed
,
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Thoma,
Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin.
1980,
Pubmed
Thomas,
The study of histone--histone associations by chemical cross-linking.
1978,
Pubmed
