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Nucleic Acids Res
1982 Dec 11;1023:7829-42. doi: 10.1093/nar/10.23.7829.
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The 3'' untranslated regions of two related mRNAs contain an element highly repeated in the sea urchin genome.
Carpenter CD
,
Bruskin AM
,
Spain LM
,
Eldon ED
,
Klein WH
.
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Two closely related cDNA clones, pSpec1 and pSpec2, specifying two developmentally regulated tissue specific mRNAs from sea urchin embryos were used to probe a sea urchin genomic lambda library. Screening 10,000 phage by plaque hybridization yielded several hundred positive signals. With more stringent wash procedures, only two to three phage were positive. Three of these phage, one isolated by stringent wash procedures and two isolated by standard wash procedures were further investigated by restriction analysis, RNA gel blots, and DNA sequencing. The phage isolated by the stringent wash procedure appears to be a gene coding for the Specl mRNA. The other phage contain only partial homology to pSpec1 and pSpec2, 150 to 200 base pairs of the 3'' untranslated region of the Spec1 and Spec2 mRNAs. It is concluded that the Spec1 and Spec2 mRNAs contain a highly repetitive element near their 3'' end. The element is present at 2000 to 3000 copies per genome and may be transcribed at some sites other than those coding for the Spec1 and Spec2 genes. The possible function and evolutionary origin of the repetitive element is discussed.
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977, Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Britten,
Gene regulation for higher cells: a theory.
1969,
Pubmed
Bruskin,
Accumulation in embryogenesis of five mRNAs enriched in the ectoderm of the sea urchin pluteus.
1981,
Pubmed
,
Echinobase
Calabretta,
mRNA in human cells contains sequences complementary to the Alu family of repeated DNA.
1982,
Pubmed
Campo,
Two classes of messenger RNA in cultured rat cells: repetitive sequence transcripts and unique sequence transcripts.
1975,
Pubmed
Costantini,
Message sequences and short repetitive sequences are interspersed in sea urchin egg poly(A)+ RNAs.
1981,
Pubmed
,
Echinobase
Davidson,
Regulation of gene expression: possible role of repetitive sequences.
1979,
Pubmed
,
Echinobase
Dina,
Relative positions of the "repetitive", "unique" and poly(A) fragments of mRNA.
1974,
Pubmed
Doolittle,
Selfish genes, the phenotype paradigm and genome evolution.
1980,
Pubmed
Galau,
Structural gene sets active in embryos and adult tissues of the sea urchin.
1976,
Pubmed
,
Echinobase
Haynes,
Low molecular weight RNAs transcribed in vitro by RNA polymerase III from Alu-type dispersed repeats in Chinese hamster DNA are also found in vivo.
1982,
Pubmed
Hough,
Sequence complexity of heterogeneous nuclear RNA in sea urchin embryos.
1975,
Pubmed
,
Echinobase
Kimmel,
A family of short, interspersed repeat sequences at the 5' end of a set of Dictyostelium single-copy mRNAs.
1979,
Pubmed
Klein,
Distribution of repetitive and nonrepetivite sequence transcripts in HeLa mRNA.
1974,
Pubmed
Klein,
Characteristics of individual repetitive sequence families in the sea urchin genome studied with cloned repeats.
1978,
Pubmed
,
Echinobase
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Moormann,
An unusually long non-coding region in rat lens alpha-crystallin messenger RNA.
1982,
Pubmed
Padgett,
Properties of dispersed, highly repeated DNA within and near the hamster CAD gene.
1982,
Pubmed
Pearson,
Repeated DNA sequences near the 5'-end of the silk fibroin gene.
1981,
Pubmed
Posakony,
Repetitive sequences of the sea urchin genome. III. Nucleotide sequences of cloned repeat elements.
1982,
Pubmed
,
Echinobase
Scheller,
Repetitive sequences of the sea urchin genome. II. Subfamily structure and evolutionary conservation.
1982,
Pubmed
,
Echinobase
Scheller,
Organization and expression of multiple actin genes in the sea urchin.
1997,
Pubmed
,
Echinobase
Tchurikov,
A cloned unique gene of Drosophila melanogaster contains a repetitive 3' exon whose sequence is present at the 3' ends of many different mRNAs.
1982,
Pubmed
Thomas,
Molecular structure of maternal RNA.
1982,
Pubmed
,
Echinobase
Tsukada,
Repetitive DNA sequences in the human corticotropin-beta-lipotrophin precursor gene region: Alu family members.
1982,
Pubmed
Wahli,
Vitellogenesis and the vitellogenin gene family.
1981,
Pubmed
Wold,
Sea urchin embryo mRNA sequences expressed in the nuclear RNA of adult tissues.
1978,
Pubmed
,
Echinobase
Zuker,
Repetitive DNA sequences cotranscribed with developmentally regulated Dictyostelium discoideum mRNAs.
1982,
Pubmed