Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Histochemistry
1989 Jan 01;926:523-9. doi: 10.1007/bf00524764.
Show Gene links
Show Anatomy links
Quantitative X-ray microanalysis of calcium in sea urchin eggs after quick-freezing and freeze-substitution. Validity of the method.
Gillot I
,
Ciapa B
,
Payan P
,
De Renzis G
,
Nicaise G
,
Sardet C
.
???displayArticle.abstract???
Freeze-substitution was used to study the distribution of calcium in sea urchin eggs, and the validity of the technique was assessed. We followed the fate of both total and exchangeable calcium of sea urchin eggs in two species (Paracentrotus lividus and Arbacia lixula) after the various treatments needed for freeze-substitution and embedding. We compared the calcium content either by X-ray microanalysis of Epon-embedded sections of freeze-substituted eggs (6.2 +/- 0.71 mmoles/kg of Epon-embedded tissue) or by flame spectrometry analysis of living eggs (32.3 +/- 1.30 nmoles/mg protein). After standardization of units, both values lead to similar total calcium content. We also measured the movements of 45Ca from prelabelled eggs. Exchangeable 45Ca as well as total calcium appeared unaffected by the preparative treatment for X-ray microanalysis. In conclusion, our preparative technique for X-ray microanalysis can be considered appropriate for our material and allows us to undertake a subcellular quantification of calcium in various organelles.
Azarnia,
The role of divalent cations in activation of the sea urchin egg. I. Effect of fertilization on divalent cation content.
1976, Pubmed,
Echinobase
Azarnia,
The role of divalent cations in activation of the sea urchin egg. I. Effect of fertilization on divalent cation content.
1976,
Pubmed
,
Echinobase
Blaineau,
Quantitative X-ray microanalysis of calcium with the Camebax-TEM system in frozen, freeze-substituted and resin-embedded tissue sections. Application to molluscan glio-interstitial granules.
1988,
Pubmed
Carafoli,
Intracellular calcium homeostasis.
1987,
Pubmed
Cardasis,
Ultrastructural localization of calcium in unfertilized sea-urchin eggs.
1978,
Pubmed
,
Echinobase
Ciapa,
Structural modifications induced by TPA (12-O-tetradecanoyl phorbol-13-acetate) in sea urchin eggs.
1988,
Pubmed
,
Echinobase
Geyer,
Ultrahistochemical demonstration of calcium ions by a freeze-substitution method.
1974,
Pubmed
Hall,
Biological X-ray microanalysis.
1979,
Pubmed
Jaffe,
Sources of calcium in egg activation: a review and hypothesis.
1983,
Pubmed
,
Echinobase
MONROY-ODDO,
Variations in Ca and Mg contents in Arbacia eggs as a result of fertilization.
2010,
Pubmed
,
Echinobase
Nicaise,
X-ray microanalysis of calcium containing organelles in resin embedded tissue.
1989,
Pubmed
Ornberg,
A freeze-substitution method for localizing divalent cations: examples from secretory systems.
1980,
Pubmed
Payan,
Na+ movements and their oscillations during fertilization and the cell cycle in sea urchin eggs.
1981,
Pubmed
,
Echinobase
Payan,
Uptake and release of calcium by isolated egg cortices of the sea urchin Paracentrotus lividus.
1987,
Pubmed
,
Echinobase
Poenie,
Ultrastructural localization of intracellular calcium stores by a new cytochemical method.
1987,
Pubmed
,
Echinobase
Sardet,
A marker of animal-vegetal polarity in the egg of the sea urchin Paracentrotus lividus. The pigment band.
1985,
Pubmed
,
Echinobase
Wilson,
The effects of salines and fixatives upon the size of an identified neuron.
1983,
Pubmed