ECB-ART-32409
Gamete Res
1987 Dec 01;184:339-48. doi: 10.1002/mrd.1120180408.
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A cortical granule-specific enzyme, B-1,3-glucanase, in sea urchin eggs.
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The ultrastructural localization of B-1,3-glucanase in three species of sea urchin eggs was determined using a monospecific antibody in an electronmicroscopic immunogold procedure. In all three species, Lytechinus variegatus, Strongylocentrotus purpuratus, and Arbacia punctulata, B-1,3-glucanase was localized specifically to the cortical granules. No other organelle within the egg contained significant label. During the fertilization reaction, B-1,3-glucanase was released from cortical granules into the perivitelline space and became associated with the hyaline layer. No significant label was found in association with the fertilization envelope.
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Genes referenced: LOC100887844
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