Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Echinobase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Echinobase
ECB-ART-41608
Bioorg Med Chem 2010 Jun 01;1811:3720-5. doi: 10.1016/j.bmc.2010.03.062.
Show Gene links Show Anatomy links

Synthesis and binding analysis of unique AG2 pentasaccharide to human Siglec-2 using NMR techniques.

Hanashima S , Sato K , Naito Y , Takematsu H , Kozutsumi Y , Ito Y , Yamaguchi Y .


???displayArticle.abstract???
Siglec-2 is a mammalian sialic acid binding protein expressed on B-cell surfaces and is involved in the modulation of B-cell mediated immune response. We synthesized a unique starfish ganglioside, AG2 pentasaccharide Galfbeta(1-3)Galpalpha(1-4)Neu5Acalpha(2-3)Galpbeta(1-4)Glcp, and found that the synthetic pentasaccharide binds to human Siglec-2 by performing (1)H NMR experiments. Saturation transfer difference NMR experiments indicated that the C7-C9 side-chain and the acetamide moiety of the central sialic acid residue were located in the binding face of human Siglec-2. We determined the binding epitope of AG2 pentasaccharide to human Siglec-2, as the Galpalpha(1-4)Neu5Acalpha(2-3)Galp unit.

???displayArticle.pubmedLink??? 20409718
???displayArticle.link??? Bioorg Med Chem


Genes referenced: LOC115919910 LOC593824