ECB-ART-30354
Biol Bull
1977 Feb 01;1521:51-63. doi: 10.2307/1540726.
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Development of Amphioplus abditus (Verrill) (Echinodermata: Ophiuroidea): I. Larval biology.
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A fraction rich in secretory granules was prepared from the pyloric caeca of Asterias amurensis by sucrose density gradient centrifugation. The freshly prepared fraction exhibited no casein-hydrolyzing activity- but showed nine times as much specific activity as that of tissue homogenates after incubation at 37 degrees C for thirty minutes. Electron microscopy showed that the secretory granules were membrane-bound granules measuring 0.5-290 mu in diameter and contained dense and/or light amorphous substances.
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