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Control mechanisms of the cell cycle: role of the spatial arrangement of spindle components in the timing of mitotic events.
Sluder G
,
Begg DA
.
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To characterize the control mechanisms for mitosis, we studied the relationship between the spatial organization of microtubules in the mitotic spindle and the timing of mitotic events. Spindles of altered geometry were produced in sea urchin eggs by two methods: (a) early prometaphase spindles were cut into half spindles by micromanipulation or (b) mercaptoethanol was used to indirectly induce the formation of spindles with only one pole. Cells with monopolar spindles produced by either method required an average of 3 X longer than control cells to traverse mitosis. By the time the control cells started their next mitosis, the experimental cells were usually just finishing the original mitosis. In all cases, only the time from nuclear envelope breakdown to the start of telophase was prolonged. Once the cells entered telophase, events leading to the next mitosis proceeded with normal timing. Once prolonged, the cell cycle never resynchronized with the controls. Several types of control experiments showed that were not an artifact of the experimental techniques. These results show that the spatial arrangement of spindle components plays an important role in the mechanisms that control the timing of mitotic events and the timing of the cell cycle as a whole.
Bajer,
Functional autonomy of monopolar spindle and evidence for oscillatory movement in mitosis.
1982, Pubmed
Bajer,
Functional autonomy of monopolar spindle and evidence for oscillatory movement in mitosis.
1982,
Pubmed
Begg,
Micromanipulation studies of chromosome movement. II. Birefringent chromosomal fibers and the mechanical attachment of chromosomes to the spindle.
1979,
Pubmed
Begg,
Micromanipulation studies of chromosome movement. I. Chromosome-spindle attachment and the mechanical properties of chromosomal spindle fibers.
1979,
Pubmed
Ellis,
Piezoelectric Micromanipulators: Electrically operated micromanipulators add automatic high-speed movement to normal manual control.
2010,
Pubmed
HINEGARDNER,
THE DNA SYNTHETIC PERIOD DURING EARLY DEVELOPMENT OF THE SEA URCHIN EGG.
1996,
Pubmed
,
Echinobase
Johnson,
Nucleo-cytoplasmic interactions in the acheivement of nuclear synchrony in DNA synthesis and mitosis in multinucleate cells.
1972,
Pubmed
Kauffman,
The mitotic oscillator in Physarum polycephalum.
1976,
Pubmed
Kinoshita,
The behaviour and localization of intracellular relaxing system during cleavage in the sea urchin egg.
1967,
Pubmed
,
Echinobase
Mazia,
Cooperation of kinetochores and pole in the establishment of monopolar mitotic apparatus.
1981,
Pubmed
,
Echinobase
REBHUN,
Aster-associated particles in the cleavage of marine invertebrate eggs.
1998,
Pubmed
Salmon,
Compensator transducer increases ease, accuracy, and rapidity of measuring changes in specimen birefringence with polarization microscopy.
1976,
Pubmed
Salmon,
Spindle microtubules: thermodynamics of in vivo assembly and role in chromosome movement.
1975,
Pubmed
,
Echinobase
Silver,
Isolation of mitotic apparatus containing vesicles with calcium sequestration activity.
1980,
Pubmed
,
Echinobase
Sluder,
Experimental manipulation of the amount of tubulin available for assembly into the spindle of dividing sea urchin eggs.
1976,
Pubmed
,
Echinobase
Sluder,
Role of spindle microtubules in the control of cell cycle timing.
1979,
Pubmed
,
Echinobase
Wolniak,
Ionic changes in the mitotic apparatus at the metaphase/anaphase transition.
1983,
Pubmed