Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Proc Natl Acad Sci U S A
1981 Nov 01;7811:6803-7. doi: 10.1073/pnas.78.11.6803.
Show Gene links
Show Anatomy links
Modulation of nucleosome structure by histone subtypes in sea urchin embryos.
Simpson RT
.
???displayArticle.abstract???
Switches of the types of histones synthesized and incorporated into chromatin occur during sea urchin embryogenesis. In an attempt to define the possible effects of these variant histones on chromatin structure, I have isolated and characterized nucleosome core particles from Strongylocentrotus purpuratus blastula (nearly 100% early histones) and pluteus (75% late histones). Both particles contain 146-base-pair lengths of DNA wrapped around an octamer of H2A, H2B, H3, and H4. Although sharing these similarities with the canonical core particle, the nucleosome structures have certain features that differ from those of typical adult tissues. Both the reversible and the irreversible conformational transitions occurring on heating core particles are destabilized in the embryonic particles vs. "typical" core particles. The blastula core particle unfolds more easily than pluteus (or other) nucleosomes under the stress of low ionic strength. The rate of DNase I digestion of pluteus core particles is about half that of particles from blastula; certain cutting sites differ in their susceptibility between the two embryonic particles and between these two and the canonical core particle. The data demonstrate that the variant histones synthesized during early embryogenesis have demonstrable effects on chromatin structure, even at this basic level.
Alfageme,
Histones of Drosophila embryos. Electrophoretic isolation and structural studies.
1974, Pubmed
Alfageme,
Histones of Drosophila embryos. Electrophoretic isolation and structural studies.
1974,
Pubmed
Arceci,
Histone variants and chromatin structure during sea urchin development.
1981,
Pubmed
,
Echinobase
Childs,
Histone gene expression during sea urchin embryogenesis: isolation and characterization of early and late messenger RNAs of Strongylocentrotus purpuratus by gene-specific hybridization and template activity.
1980,
Pubmed
,
Echinobase
Cohen,
Stage-specific switches in histone synthesis during embryogenesis of the sea urchin.
1976,
Pubmed
,
Echinobase
Gordon,
Conformational changes of the chromatin subunit.
1978,
Pubmed
Gordon,
The temperature and pH dependence of conformational transitions of the chromatin subunit.
1979,
Pubmed
Gross,
Cell-free translation of maternal messenger RNA from sea urchin eggs.
1973,
Pubmed
,
Echinobase
Isenberg,
Histones.
1979,
Pubmed
Keichline,
Structure of chromatin in sea urchin embryos, sperm, and adult somatic cells.
1979,
Pubmed
,
Echinobase
Keichline,
Developmental study of the structure of sea urchin embryo and sperm chromatin using micrococcal nuclease.
1977,
Pubmed
,
Echinobase
Leffak,
Conservative assembly and segregation of nucleosomal histones.
1978,
Pubmed
Lutter,
Kinetic analysis of deoxyribonuclease I cleavages in the nucleosome core: evidence for a DNA superhelix.
1979,
Pubmed
Mardian,
Nucleosome cores have two specific binding sites for nonhistone chromosomal proteins HMG 14 and HMG 17.
1981,
Pubmed
McCarty,
Improved computer program data for the resolution and fractionation of macromolecules by isokinetic sucrose density gradient sedimentation.
1974,
Pubmed
McGhee,
Nucleosome structure.
1980,
Pubmed
McGhee,
Orientation of the nucleosome within the higher order structure of chromatin.
1981,
Pubmed
Newrock,
Stage-specific mRNAs coding for subtypes of H2A and H2B histones in the sea urchin embryo.
1978,
Pubmed
,
Echinobase
Newrock,
Histone changes during chromatin remodeling in embryogenesis.
1978,
Pubmed
,
Echinobase
Noll,
DNA folding in the nucleosome.
1978,
Pubmed
Ruderman,
Histones and histone synthesis in sea urchin development.
1974,
Pubmed
,
Echinobase
Sandeen,
The interaction of high mobility proteins HMG14 and 17 with nucleosomes.
1981,
Pubmed
Seale,
Chromatin-associated proteins of the developing sea urchin embryo. I. Kinetics of synthesis and characterization of non-histone proteins.
1973,
Pubmed
,
Echinobase
Simpson,
Structure of chromatin containing extensively acetylated H3 and H4.
1978,
Pubmed
Simpson,
Cromatin and core particles formed from the inner histones and synthetic polydeoxyribonucleotides of defined sequence.
1979,
Pubmed
Simpson,
Mechanism of a reversible, thermally induced conformational change in chromatin core particles.
1979,
Pubmed
Simpson,
Conformation of DNA in chromatin core particles containing poly(dAdT)-poly(dAdT) studied by 31 P NMR spectroscopy.
1979,
Pubmed
Simpson,
Structure of sea urchin sperm chromatin core particle.
1981,
Pubmed
,
Echinobase
Simpson,
Structure of the chromatosome, a chromatin particle containing 160 base pairs of DNA and all the histones.
1979,
Pubmed
Simpson,
Mapping DNAase l-susceptible sites in nucleosomes labeled at the 5' ends.
1976,
Pubmed
Skoultchi,
Maternal histone messenger RNA: detection by molecular hybridization.
1973,
Pubmed
,
Echinobase
Sures,
The DNA sequence of sea urchin (S. purpuratus) H2A, H2B and H3 histone coding and spacer regions.
1979,
Pubmed
,
Echinobase
Weischet,
Thermal denaturation of nucleosomal core particles.
1978,
Pubmed
Whitlock,
Preparation and physical characterization of a homogeneous population of monomeric nucleosomes from HeLa cells.
1976,
Pubmed
