ECB-ART-37501
Biosci Biotechnol Biochem
2000 Jun 01;646:1278-81. doi: 10.1271/bbb.64.1278.
Show Gene links
Show Anatomy links
Effects of chemical modification of carboxyl groups in the hemolytic lectin CEL-III on its hemolytic and carbohydrate-binding activities.
???displayArticle.abstract???
Effects of chemical modification of carboxyl groups in the hemolytic lectin CEL-III on its activities were investigated. When carboxyl groups were modified with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and glycine methyl ester, hemolytic activity of CEL-III decreased as the EDC concentration increased, accompanied by reduction of oligomerization ability and hemagglutinating activity. However, binding ability of CEL-III for immobilized lactose was retained fairly well after modification, suggesting that one of two carbohydrate-binding sites might be responsible for such inactivation of CEL-III.
???displayArticle.pubmedLink??? 10923802
???displayArticle.link??? Biosci Biotechnol Biochem