ECB-ART-40944
Biochim Biophys Acta
2009 Jan 01;17871:15-24. doi: 10.1016/j.bbabio.2008.10.003.
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Mitochondrial inhibitors activate influx of external Ca(2+) in sea urchin sperm.
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Sea urchin sperm have a single mitochondrion which, aside from its main ATP generating function, may regulate motility, intracellular Ca(2+) concentration ([Ca(2+)](i)) and possibly the acrosome reaction (AR). We have found that acute application of agents that inhibit mitochondrial function via differing mechanisms (CCCP, a proton gradient uncoupler, antimycin, a respiratory chain inhibitor, oligomycin, a mitochondrial ATPase inhibitor and CGP37157, a Na(+)/Ca(2+) exchange inhibitor) increases [Ca(2+)](i) with at least two differing profiles. These increases depend on the presence of extracellular Ca(2+), which indicates they involve Ca(2+) uptake and not only mitochondrial Ca(2+) release. The plasma membrane permeation pathways activated by the mitochondrial inhibitors are permeable to Mn(2+). Store-operated Ca(2+) channel (SOC) blockers (Ni(2+), SKF96365 and Gd(2+)) and internal-store ATPase inhibitors (thapsigargin and bisphenol) antagonize Ca(2+) influx induced by the mitochondrial inhibitors. The results indicate that the functional status of the sea urchin sperm mitochondrion regulates Ca(2+) entry through SOCs. As neither CCCP nor dicycloexyl carbodiimide (DCCD), another mitochondrial ATPase inhibitor, eliminate the oligomycin induced increase in [Ca(2+)](i), apparently oligomycin also has an extra mitochondrial target.
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Genes referenced: LOC100887844 LOC581395