ECB-ART-43629
Methods Mol Biol
2015 Jan 01;1189:283-97. doi: 10.1007/978-1-4939-1164-6_19.
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Primary cell cultures of regenerating holothurian tissues.
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The ability to culture different cell types is essential for answering many questions in developmental and regenerative biology. Studies in marine organisms, in particular echinoderms, have been limited by the lack of well-described cellular culture systems. Here we describe a cell culture system, for normal or regenerating holothurian cells, that allows cell characterization by immunohistochemistry and scanning electron microscopy. These cell cultures can now be used to perform multiple types of experiments in order to explore the cellular, biochemical, and genomic aspects of echinoderm regenerative properties.
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Genes referenced: LOC115919910
References [+] :
Candia Carnevali,
Cellular and molecular mechanisms of arm regeneration in crinoid echinoderms: the potential of arm explants.
1998, Pubmed,
Echinobase