Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Braz J Microbiol
2015 Jun 01;462:347-54. doi: 10.1590/S1517-838246220130316.
Show Gene links
Show Anatomy links
Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts.
Oliveira LC
,
Ramos PL
,
Marem A
,
Kondo MY
,
Rocha RC
,
Bertolini T
,
Silveira MA
,
da Cruz JB
,
de Vasconcellos SP
,
Juliano L
,
Okamoto DN
.
???displayArticle.abstract???
Halophilic microorganisms are able to grow in the presence of salt and are also excellent source of enzymes and biotechnological products, such as exopolysaccharides (EPSs) and polyhydroxyalkanoates (PHAs). Salt-tolerant bacteria were screened in the Organic Composting Production Unit (OCPU) of São Paulo Zoological Park Foundation, which processes 4 ton/day of organic residues including plant matter from the Atlantic Rain Forest, animal manure and carcasses and mud from water treatment. Among the screened microorganisms, eight halotolerant bacteria grew at NaCl concentrations up to 4 M. These cultures were classified based on phylogenetic characteristics and comparative partial 16S rRNA gene sequence analysis as belonging to the genera Staphylococcus, Bacillus and Brevibacterium. The results of this study describe the ability of these halotolerant bacteria to produce some classes of hydrolases, namely, lipases, proteases, amylases and cellulases, and biopolymers. The strain characterized as of Brevibacterium avium presented cellulase and amylase activities up to 4 M NaCl and also produced EPSs and PHAs. These results indicate the biotechnological potential of certain microorganisms recovered from the composting process, including halotolerant species, which have the ability to produce enzymes and biopolymers, offering new perspectives for environmental and industrial applications.
Figure 1. Partial view of the Organic Composting Production Unit (OCPU) of
São Paulo Zoological Park Foundation. (A) Aerobic composting process
in the thermophilic phase; (B) grinding of plant matter from the
Atlantic Rain Forest; (C) maturation phase of the composting
process.
Figure 2. Phylogenetic tree showing the position of the halotolerant isolates,
as based on a partial 16S rRNA gene sequence comparison obtained by
neighbor-joining and maximum-likelihood trees. The nucleotide sequence
accession numbers were deposited in GenBank, as described in Material
and Methods.
Chen,
Bacillus hemicentroti sp. nov., a moderate halophile isolated from a sea urchin.
2012, Pubmed,
Echinobase
Chen,
Bacillus hemicentroti sp. nov., a moderate halophile isolated from a sea urchin.
2012,
Pubmed
,
Echinobase
Chen,
Bacillus hunanensis sp. nov., a slightly halophilic bacterium isolated from non-saline forest soil.
2011,
Pubmed
Cho,
Bacillus chungangensis sp. nov., a halophilic species isolated from sea sand.
2010,
Pubmed
Chun,
A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences.
1995,
Pubmed
Dojka,
Microbial diversity in a hydrocarbon- and chlorinated-solvent-contaminated aquifer undergoing intrinsic bioremediation.
1998,
Pubmed
Essghaier,
Biological control of grey mould in strawberry fruits by halophilic bacteria.
2010,
Pubmed
Fuciños,
Lipases and esterases from extremophiles: overview and case example of the production and purification of an esterase from Thermus thermophilus HB27.
2012,
Pubmed
Gupta,
Bacterial alkaline proteases: molecular approaches and industrial applications.
2002,
Pubmed
Legat,
Identification of polyhydroxyalkanoates in Halococcus and other haloarchaeal species.
2010,
Pubmed
Li,
Extracellular production of beta-amylase by a halophilic isolate, Halobacillus sp. LY9.
2012,
Pubmed
Litchfield,
Potential for industrial products from the halophilic Archaea.
2012,
Pubmed
Martins,
Metagenomic analysis of a tropical composting operation at the são paulo zoo park reveals diversity of biomass degradation functions and organisms.
2013,
Pubmed
Nagata,
Efficient utilization of ectoine by halophilic Brevibacterium species and Escherichia coli subjected to osmotic downshock.
2005,
Pubmed
Nübel,
Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis.
1996,
Pubmed
Pappa,
Bacillus halochares sp. nov., a halophilic bacterium isolated from a solar saltern.
2010,
Pubmed
Partanen,
Bacterial diversity at different stages of the composting process.
2010,
Pubmed
Pascon,
Amylolytic microorganism from são paulo zoo composting: isolation, identification, and amylase production.
2011,
Pubmed
,
Echinobase
Poli,
Synthesis, production, and biotechnological applications of exopolysaccharides and polyhydroxyalkanoates by archaea.
2012,
Pubmed
Ram Kumar Pandian,
Synthesis of PHB nanoparticles from optimized medium utilizing dairy industrial waste using Brevibacterium casei SRKP2: a green chemistry approach.
2009,
Pubmed
Rohban,
Screening and isolation of halophilic bacteria producing extracellular hydrolyses from Howz Soltan Lake, Iran.
2009,
Pubmed
Ron,
Biosurfactants and oil bioremediation.
2003,
Pubmed
Schlegel,
The isolation of mutants not accumulating poly-beta-hydroxybutyric acid.
1970,
Pubmed
Schloss,
Tracking temporal changes of bacterial community fingerprints during the initial stages of composting.
2012,
Pubmed
Sánchez-Porro,
Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes.
2003,
Pubmed
Takenaka,
Molecular cloning and sequence analysis of two distinct halotolerant extracellular proteases from Bacillus subtilis FP-133.
2011,
Pubmed
Tanasupawat,
Identification of moderately halophilic bacteria from Thai fermented fish ( pla-ra ) and proposal of Virgibacillus siamensis sp. nov.
2011,
Pubmed
Vasconcellos,
The potential for hydrocarbon biodegradation and production of extracellular polymeric substances by aerobic bacteria isolated from a Brazilian petroleum reservoir.
2014,
Pubmed
,
Echinobase
Ventosa,
Biotechnological applications and potentialities of halophilic microorganisms.
2014,
Pubmed
Ventosa,
Biology of moderately halophilic aerobic bacteria.
1998,
Pubmed
Voget,
Characterization of a metagenome-derived halotolerant cellulase.
2006,
Pubmed
Wang,
The biodegradation of 1,3-dichlorobenzene by an adapted strain Bacillus cereus PF-11 derived from town-gas industrial effluent.
2003,
Pubmed
Wong,
Microbial production of polyhydroxyalkanoates by bacteria isolated from oil wastes.
2000,
Pubmed
Zhang,
Effects of salts on activity of halophilic cellulase with glucomannanase activity isolated from alkaliphilic and halophilic Bacillus sp. BG-CS10.
2012,
Pubmed