Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Lipids
2017 Feb 01;522:119-127. doi: 10.1007/s11745-016-4222-1.
Show Gene links
Show Anatomy links
Eicosapentaenoic Acid-Enriched Phosphatidylcholine Attenuated Hepatic Steatosis Through Regulation of Cholesterol Metabolism in Rats with Nonalcoholic Fatty Liver Disease.
Liu Y
,
Shi D
,
Tian Y
,
Liu Y
,
Zhan Q
,
Xu J
,
Wang J
,
Xue C
.
???displayArticle.abstract???
Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in the world. Disturbed cholesterol metabolism plays a crucial role in the development of NAFLD. The present study was conducted to evaluate the effects of EPA-PC extracted from sea cucumber on liver steatosis and cholesterol metabolism in NAFLD. Male Wistar rats were randomly divided into seven groups (normal control group, model group, lovastatin group, low- and high-dose EPA groups, and low- and high-dose EPA-PC groups). Model rats were established by administering a diet containing 1% orotic acid. To determine the possible cholesterol metabolism promoting mechanism of EPA-PC, we analyzed the transcription of key genes and transcriptional factors involved in hepatic cholesterol metabolism. EPA-PC dramatically alleviated hepatic lipid accumulation, reduced the serum TC concentration, and elevated HDLC levels in NAFLD rats. Fecal neutral cholesterol excretion was also promoted by EPA-PC administration. Additionally, EPA-PC decreased the mRNA expression of hydroxymethyl glutaric acid acyl (HMGR) and cholesterol 7α-hydroxylase (CYP7A), and increased the transcription of sterol carrying protein 2 (SCP2). Moreover, EPA-PC stimulated the transcription of peroxisome proliferators-activated receptor α (PPARα) and adenosine monophosphate activated protein kinase (AMPK) as well as its modulators, liver kinase B1 (LKB1) and Ca2+/calmodulin-dependent kinase kinase (CAMKK). Based on the results, the promoting effects of EPA-PC on NAFLD may be partly associated with the suppression of cholesterol synthesis via HMGR inhibition and the enhancement of fecal cholesterol excretion through increased SCP2 transcription. The underlying mechanism may involve stimulation of PPARα and AMPK.
Agellon,
The 3'-untranslated region of the mouse cholesterol 7alpha-hydroxylase mRNA contains elements responsive to post-transcriptional regulation by bile acids.
1998, Pubmed
Agellon,
The 3'-untranslated region of the mouse cholesterol 7alpha-hydroxylase mRNA contains elements responsive to post-transcriptional regulation by bile acids.
1998,
Pubmed
Baker,
One or more labile proteins regulate the stability of chimeric mRNAs containing the 3'-untranslated region of cholesterol-7alpha -hydroxylase mRNA.
2000,
Pubmed
Burg,
Regulation of HMG-CoA reductase in mammals and yeast.
2012,
Pubmed
Caballero,
Enhanced free cholesterol, SREBP-2 and StAR expression in human NASH.
2009,
Pubmed
Chen,
Fenofibrate lowers lipid accumulation in myotubes by modulating the PPARα/AMPK/FoxO1/ATGL pathway.
2012,
Pubmed
Christians,
Metabolism and drug interactions of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors in transplant patients: are the statins mechanistically similar?
1999,
Pubmed
DeFilippis,
Nonalcoholic fatty liver disease and serum lipoproteins: the Multi-Ethnic Study of Atherosclerosis.
2013,
Pubmed
Du,
Dietary eicosapentaenoic acid supplementation accentuates hepatic triglyceride accumulation in mice with impaired fatty acid oxidation capacity.
2013,
Pubmed
FOLCH,
A simple method for the isolation and purification of total lipides from animal tissues.
2002,
Pubmed
Fullerton,
Immunometabolism of AMPK in insulin resistance and atherosclerosis.
2013,
Pubmed
Gilardi,
The pharmacological exploitation of cholesterol 7alpha-hydroxylase, the key enzyme in bile acid synthesis: from binding resins to chromatin remodelling to reduce plasma cholesterol.
2008,
Pubmed
Hamaguchi,
Nonalcoholic fatty liver disease is a novel predictor of cardiovascular disease.
2007,
Pubmed
Hossain,
Docosahexaenoic acid and eicosapentaenoic acid-enriched phosphatidylcholine liposomes enhance the permeability, transportation and uptake of phospholipids in Caco-2 cells.
2006,
Pubmed
Hsu,
Monascin and ankaflavin act as natural AMPK activators with PPARα agonist activity to down-regulate nonalcoholic steatohepatitis in high-fat diet-fed C57BL/6 mice.
2014,
Pubmed
Hu,
Eicosapentaenoic acid-enriched phosphatidylcholine isolated from Cucumaria frondosa exhibits anti-hyperglycemic effects via activating phosphoinositide 3-kinase/protein kinase B signal pathway.
2014,
Pubmed
,
Echinobase
Hunt,
The peroxisome proliferator-activated receptor alpha (PPARalpha) regulates bile acid biosynthesis.
2000,
Pubmed
Jung,
n-3 fatty acids ameliorate hepatic steatosis and dysfunction after LXR agonist ingestion in mice.
2011,
Pubmed
Krawczyk,
Nonalcoholic fatty liver disease.
2011,
Pubmed
Lefebvre,
Role of bile acids and bile acid receptors in metabolic regulation.
2009,
Pubmed
Liu,
Eicosapentaenoic acid-enriched phospholipid ameliorates insulin resistance and lipid metabolism in diet-induced-obese mice.
2013,
Pubmed
Loguercio,
Silybin combined with phosphatidylcholine and vitamin E in patients with nonalcoholic fatty liver disease: a randomized controlled trial.
2012,
Pubmed
Lorbek,
Cytochrome P450s in the synthesis of cholesterol and bile acids--from mouse models to human diseases.
2012,
Pubmed
Min,
Increased hepatic synthesis and dysregulation of cholesterol metabolism is associated with the severity of nonalcoholic fatty liver disease.
2012,
Pubmed
Moncecchi,
Sterol carrier protein-2 expression in mouse L-cell fibroblasts alters cholesterol uptake.
1996,
Pubmed
Murphy,
Sterol carrier protein-2 mediated cholesterol esterification in transfected L-cell fibroblasts.
1997,
Pubmed
Musso,
Cholesterol metabolism and the pathogenesis of non-alcoholic steatohepatitis.
2013,
Pubmed
Nomura,
The role of fructose-enriched diets in mechanisms of nonalcoholic fatty liver disease.
2012,
Pubmed
Oakhill,
AMPK functions as an adenylate charge-regulated protein kinase.
2012,
Pubmed
Schroeder,
Sterol carrier protein-2: new roles in regulating lipid rafts and signaling.
2007,
Pubmed
Seedorf,
Sterol carrier protein-2.
2000,
Pubmed
Sun,
Osthol regulates hepatic PPAR alpha-mediated lipogenic gene expression in alcoholic fatty liver murine.
2010,
Pubmed
Targher,
Risk of cardiovascular disease in patients with nonalcoholic fatty liver disease.
2010,
Pubmed
Targher,
Nonalcoholic fatty liver disease is independently associated with an increased incidence of cardiovascular events in type 2 diabetic patients.
2007,
Pubmed
Wang,
Artemisia scoparia extract attenuates non-alcoholic fatty liver disease in diet-induced obesity mice by enhancing hepatic insulin and AMPK signaling independently of FGF21 pathway.
2013,
Pubmed
Wang,
Study on possible mechanism of orotic acid-induced fatty liver in rats.
2011,
Pubmed
West,
Lipid structure does not modify incorporation of EPA and DHA into blood lipids in healthy adults: a randomised-controlled trial.
2017,
Pubmed
Witczak,
AMP-activated protein kinase in skeletal muscle: from structure and localization to its role as a master regulator of cellular metabolism.
2009,
Pubmed
Xu,
Medium-chain fatty acids enhanced the excretion of fecal cholesterol and cholic acid in C57BL/6J mice fed a cholesterol-rich diet.
2014,
Pubmed
Xu,
Isolation and anti-fatty liver activity of a novel cerebroside from the sea cucumber Acaudina molpadioides.
2012,
Pubmed
,
Echinobase
Yu,
Structural study of fucoidan from sea cucumber Acaudina molpadioides: a fucoidan containing novel tetrafucose repeating unit.
2014,
Pubmed
,
Echinobase