ECB-ART-47772
FEBS Lett
2002 Aug 14;5251-3:20-4. doi: 10.1016/s0014-5793(02)03000-4.
Show Gene links
Show Anatomy links
A caged sperm-activating peptide that has a photocleavable protecting group on the backbone amide.
???displayArticle.abstract???
A backbone-caged sperm-activating peptide (caged speract) that has a 2-nitrobenzyl group at a backbone amide and a vastly reduced affinity for its receptor (IC50=950 nM) was synthesized. UV irradiation of caged speract photocleaves the 2-nitrobenzyl group (tau1/2=26 micros), restoring its affinity (IC50=0.67 nM) and ability to increase sperm intracellular pH and Ca2+, as intact speract. Backbone caging of the biological activity was more efficient than side chain caging, which adds a nitrobenzyl group on the peptide side chain. The backbone caging strategy described can be used as a general procedure to cage biologically active peptides, which have no side chain for introduction of a caging group.
???displayArticle.pubmedLink??? 12163154
???displayArticle.link??? FEBS Lett
Genes referenced: LOC576642