ECB-ART-48853
Curr Biol
2020 May 18;3010:R427-R429. doi: 10.1016/j.cub.2020.03.057.
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Establishment of homozygous knock-out sea urchins.
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Yaguchi et al. establish a homozygous knock-out sea urchin line by applying the CRISPR-Cas9 system to a new model species, Temnopleurus reevesii, whose breeding cycle takes about half a year.
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Genes referenced: LOC100887844
gRNAs referenced: LOC588806 gRNA28 LOC588806 gRNA29