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ECB-ANTIBODY-26395821
Antibody Name:
LOC580458 Ab1
Common Name: sushi domain-containing protein 2 RRID: Synonyms: SpMesh, SpSusd2
Source: |
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Antibody | |
Clone Number | |
Purification | |
Isotype | IgG |
Host Organism | rabbit |
Echinoderm Reactivity | S. purpuratus, H. pulcherrimus |
Non-Echinoderm Reactivity | |
Description: | Reacts with a ~120 kDa protein that localizes to septate junctions of the midgut and hindgut. Generated by Kiwa Laboratory Animals (Wakayama, Japan) for initial usage by the Heyland lab at the University of Guelph in Canada. |
Immunogen | |
Name | HpMesh |
Type | polypeptide |
Post Translational Modifications | None |
Source Organism | Hemicentrotus pulcherrimus |
Description | Glutathione-S-transferase fusion protein generated using an amino acid-fragment representing the C-terminal cytoplasmic region, which was amplified using the forward primer, 5'-acgcgtcgacAAAACCAATAGCAGCTACCCAAGGAAATCG-3', and the reverse primer, 5'-acgcgtcgacCTAAATCCACCCACCAGTACCTTTGATGGT-3'. |
Sequence | |
Reported Usage | |
immunofluorescence | |
western blot | |
immunohistochemistry | |
Publications | |
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Identification of the genes encoding candidate septate junction components expressed during early development of the sea urchin, Strongylocentrotus purpuratus, and evidence of a role for Mesh in the formation of the gut barrier., Dev Biol 2023 |
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