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ECB-ANTIBODY-26395889
Antibody Name:
pm27 Ab1
Common Name: 27 kDa primary mesenchyme-specific spicule protein RRID: Synonyms: PM27
Source: |
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Antibody | |
Clone Number | |
Purification | Affinity Purification |
Isotype | isotype unknown |
Host Organism | rabbit |
Echinoderm Reactivity | S. purpuratus |
Non-Echinoderm Reactivity | |
Description: | Skeletogenic mesenchyme-specific antibody created and previously supplied by the laboratory of Professor Rudolph Raff at the University of Washington, Seattle. |
Immunogen | |
Name | PM27-PWR 590 and PM27-pGEX fusion proteins |
Type | polypeptide |
Post Translational Modifications | None |
Source Organism | purple sea urchin |
Description | Both fusion proteins contain the same 243 amino-acid coding region, which originated from the nucleotide sequence within the NaeI (+156) and EcoRI (+883) sites of a Bluescript SK+ vector. PM27-PWR 590 was used for the primary injection, while PM27-pGEX fusion protein was used for the final injection. |
Sequence | |
Reported Usage | |
immunohistochemistry | |
western blot | |
Publications | |
???displayAntibody.papersFirst??? |
Structure, expression, and extracellular targeting of PM27, a skeletal protein associated specifically with growth of the sea urchin larval spicule., Dev Biol 1995 |
???displayAntibody.papersRecent??? |
Expression of spicule matrix proteins in the sea urchin embryo during normal and experimentally altered spiculogenesis., Dev Biol 2000 |
???displayAntibody.papersAll??? |