Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Echinobase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Echinobase

Summary Attributions Wiki
ECB-GRNA-26395073
gRNA Name: LOC580458 gRNA1
Synonyms: Spmesh 306
Genomic Target: LOC580458
Derived from:

Sequence: 5' GGACGGGCGAATTGAGGACC 3'



Genomic Target S. purpuratus
Identities 19/20
genome
gRNA
 
5' GGTCCTCAATTCGCCCGTCG 3'
   ||||||||||||||||||| 
3' CCAGGAGTTAAGCGGGCAGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145594.1:28423591..28423610 19/20 Sense target LOC580458
NW_022145594.1:28205545..28205564 19/20 Antisense off-target LOC105439366
NW_022145594.1:28239869..28239888 19/20 Antisense off-target LOC115921309
NW_022145594.1:28319664..28319683 18/20 Antisense off-target LOC115918445
NW_022145615.1:732201..732220 18/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Optimizing CRISPR/Cas9-based gene manipulation in echinoderms., Dev Biol 2022  
???morpholino.displaySummary.papersRecent??? Identification of the genes encoding candidate septate junction components expressed during early development of the sea urchin, Strongylocentrotus purpuratus, and evidence of a role for Mesh in the formation of the gut barrier., Dev Biol 2023  
???morpholino.displaySummary.papersAll???