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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395075
gRNA Name: LOC580458 gRNA2
Synonyms: Spmesh 346
Genomic Target: LOC580458
Derived from:

Sequence: 5' GGGGCCGATGTAGATATTAC 3'



Genomic Target S. purpuratus
Identities 19/20
genome
gRNA
 
5' GTAATATCTACATCGGCCCA 3'
   ||||||||||||||||||| 
3' CATTATAGATGTAGCCGGGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145594.1:28423631..28423650 19/20 Sense target LOC580458
NW_022145614.1:31644833..31644852 18/20 Sense off-target gid8
NW_022145611.1:25350244..25350263 18/20 Sense off-target LOC581799
NW_022145594.1:34489710..34489729 18/20 Sense unknown
NW_022145598.1:34267111..34267130 18/20 Sense unknown
P. miniata 3.0 NW_024037061.1:14873864..14873883 16/20 Antisense off-target LOC119730878
L. variegatus 3.0 chr10:27064871..27064890 16/20 Antisense off-target LOC121422895
chr8:11672092..11672111 18/20 Sense unknown
chr11:11960893..11960912 17/20 Antisense unknown
chr11:27470438..27470457 17/20 Sense unknown
chr11:8061511..8061530 17/20 Antisense unknown
chr12:22899128..22899147 17/20 Sense unknown
chr1:12110619..12110638 16/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Optimizing CRISPR/Cas9-based gene manipulation in echinoderms., Dev Biol 2022  
???morpholino.displaySummary.papersRecent??? Identification of the genes encoding candidate septate junction components expressed during early development of the sea urchin, Strongylocentrotus purpuratus, and evidence of a role for Mesh in the formation of the gut barrier., Dev Biol 2023  
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